TITLE: Postdoctoral Scholar
EMAIL: stefanieluecke@nullg.ucla.edu
EDUCATION:
Ph.D. in Biomedicine, Aarhus University, Denmark, 2018
M.Sc. in Infection and Immunity, Utrecht University, The Netherlands, 2014
B.Sc. in Biochemistry and Cell Biology, Jacobs University Bremen, Germany, 2012

Research Interests

I am investigating how combinatorial and temporal codes generated by the innate immune signaling network act together to determine stimulus-specific macrophage responses.

Publications

1: Luecke S*, Sheu KM*, Hoffmann A. Immune Sentinel Cells: Molecular Mechanisms for Combinatorial and Temporal Coding to Produce Stimulus-Appropriate Responses. Immunity. 2021; 54(9):1915-1932. Review. *SL and KMS contributed equally.

2: Juul-Madsen K, Troldborg A, Wittenborn TR, Axelsen MG, Zhao H, Klausen LH, Luecke S, Paludan SR, Steengaard-Pedersen K, Dong M, Møller HJ, Thiel S, Jensen H, Schuck P, Sutherland DS, Degn SE, Vorup-Jensen T. Characterization of DNA-protein complexes by nanoparticle tracking analysis and their association with systemic lupus erythematosus. PNAS. 2021;118(30).

3: Adelaja A*, Taylor B*, Sheu KM, Liu Y, Luecke S, Hoffmann A. Six distinct NFκB signaling codons convey discrete information to distinguish stimuli and enable appropriate macrophage responses. Immunity. 2021; 54, 916-930. *AA and BT contributed equally.

4: Chitadze, G, Lettau, M, Peters, C, Luecke S, Flueh C, Quabius ES, Synowitz M, Held-Feindt J, Kabelitz D. Erroneous expression of NKG2D on granulocytes detected by phycoerythrin-conjugated clone 149810 antibody. Cytometry. 2021; 1– 11.

5: Sheu KM*, Luecke S*, Hoffmann A. Stimulus-specificity in the responses of immune sentinel cells. Curr Opin Syst Biol. 2019; 18:53-61. Review. *KMS and SL contributed equally.

6: Sun C*, Luecke S*, Bodda C, Jønsson KL, Cai Y, Zhang BC, Jensen SB, Nordentoft I, Jensen JM, Jakobsen MR, Paludan SR. Cellular Requirements for Sensing and Elimination of Incoming HSV-1 DNA and Capsids. J Interferon Cytokine Res. 2019 Mar 11. doi: 10.1089/jir.2018.0141.  *CS and SLcontributed equally

7: Chitadze G, Flüh C, Quabius ES, Freitag-Wolf S, Peters C, Lettau M, Bhat J, Wesch D, Oberg HH, Luecke S, Janssen O, Synowitz M, Held-Feindt J, Kabelitz D. In-depth immunophenotyping of patients with glioblastoma multiforme: Impact of steroid treatment. Oncoimmunology. 2017 Aug 8;6(11):e1358839. doi: 10.1080/2162402X.2017.1358839.

8: Luecke S, Holleufer A, Christensen MH, Jønsson KL, Boni GA, Sørensen LK, Johannsen M, Jakobsen MR, Hartmann R, Paludan SR. cGAS is activated by DNA in a length-dependent manner. EMBO Rep. 2017 Oct;18(10):1707-1715. doi: 10.15252/embr.201744017.

9: Ogunjimi B, Zhang SY, Sørensen KB, Skipper KA, Carter-Timofte M, Kerner G, Luecke S, Prabakaran T, Cai Y, Meester J, Bartholomeus E, Bolar NA, Vandeweyer G, Claes C, Sillis Y, Lorenzo L, Fiorenza RA, Boucherit S, Dielman C, Heynderickx S, Elias G, Kurotova A, Auwera AV, Verstraete L, Lagae L, Verhelst H, Jansen A, Ramet J, Suls A, Smits E, Ceulemans B, Van Laer L, Plat Wilson G, Kreth J, Picard C, Von Bernuth H, Fluss J, Chabrier S, Abel L, Mortier G, Fribourg S, Mikkelsen JG, Casanova JL, Paludan SR, Mogensen TH. Inborn errors in RNA polymerase III underlie severe varicella zoster virus infections. J Clin Invest. 2017 Sep 1;127(9):3543-3556. doi: 10.1172/JCI92280.

10: Luecke S, Paludan SR. Molecular requirements for sensing of intracellular microbial nucleic acids by the innate immune system. Cytokine. 2017 Oct; 98:4-14. doi: 10.1016/j.cyto.2016.10.003. Review.

11: Christensen MH, Jensen SB, Miettinen JJ, Luecke S, Prabakaran T, Reinert LS, Mettenleiter T, Chen ZJ, Knipe DM, Sandri-Goldin RM, Enquist LW, Hartmann R, Mogensen TH, Rice SA, Nyman TA, Matikainen S, Paludan SR. HSV-1 ICP27 targets the TBK1-activated STING signalsome to inhibit virus-induced type I IFN expression. EMBO J. 2016 Jul 1;35(13):1385-99. doi: 10.15252/embj.201593458.

12: Chitadze G, Lettau M, Luecke S, Wang T, Janssen O, Fürst D, Mytilineos J, Wesch D, Oberg HH, Held-Feindt J, Kabelitz D. NKG2D- and T-cell receptor-dependent lysis of malignant glioma cell lines by human γδ T cells: Modulation by temozolomide and A disintegrin and metalloproteases 10 and 17 inhibitors. Oncoimmunology. 2015 Dec 10;5(4):e1093276.

13: Luecke S, Paludan SR. Innate recognition of alphaherpesvirus DNA. Adv Virus Res. 2015;92:63-100. doi: 10.1016/bs.aivir.2014.11.003. Review.

TITLE: Graduate Student (PhD)
PROGRAM: Biochemistry, Molecular, and Structural Biology (BMSB) (2016 – present)
EMAIL: xlin16@nullucla.edu
EDUCATION:
B.A. in Biology (Honors), New York University – 2016

Biography

My research aims to use mathematical modeling in the context of high cell-to-cell variability to elucidate the network behavior of the NFκB system for applications in therapeutics. Specific aims of my project include developing novel methods for quantitatively fitting math models to single-cell data, identifying key stimulus-specific NFκB dynamics, pinpointing crucial sources of molecular noise that diminish NFκB information encoding, and understanding the mechanisms for decoding NFκB dynamics into stimulus-specific gene expression. I am particularly passionate about advocating for mental health awareness in graduate education. My hobbies include yoga and photography.

Awards & Fellowships:

UCLA Grad Slam – Audience Choice Award (2019) (Talk Title: Deciphering the Immune System Code – VIDEO HERE)
UCLA Cellular and Molecular Biology Trainee (2018-present)
NSF Graduate Research Fellowship Program (GRFP) – Honorable Mention (2018)

Follow @xiaofei_lin

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TITLE: Graduate Student (PhD)
PROGRAM: Bioinformatics (2015-present)
EMAIL: ningw2015@nullucla.edu
OFFICE: Boyer 510B
EDUCATION:
BS Electrical Engineering, University of Science and Technology of China, 2013

Research Interests

Ning is passionate about developing quantitative platform to understand and predict gene response under multiple transcription factors control in macrophage pathogen response. Ning addresses this challenge by integrating mechanistic and statistical model to abstract biological insight from high-throughput sequencing data.

TITLE: Postdoctoral Scholar
OFFICE: Boyer 570
EMAIL: koushikroy@nullucla.edu
PHONE: 310-210-4357
EDUCATION:
Ph.D. Biotechnology, CSIR-Indian Institute of Chemical Biology (2014, CU)
M.Sc. Chemistry, Jadavpur University (2006)

Research Interests

NFκB is a key regulator of B cell activation with subsequent proliferation and differentiation. cRel is necessary for B cell proliferation and RelA is necessary for B cell differentiation. Misregulated NFκB is associated with immunological disease such as B cell lymphoma.We have developed an integrated system of single cell microscopy tracking and dye dilution analysis tool to quantitatively study B cell population dynamics in terms of cell biological parameters at the single cell level. I am interested to study what is the role of dimer and the dynamic to the B cell fate decision at single cell level and population level.

Awards

Boyer/Parvin Postdoctoral Award, Recognizing Excellence in Postdoctoral Research, UCLA, 2019
Best Presentation, Preventive Medicine: Current Perspective, India, 2014
Best Presentation, CSIR-Indian Institute of Chemical Biology on Immunology day, India, 2014
Best Poster, International Symposium on Molecular Signaling, India, 2013
Best Poster, Society of Biological Chemist Symposium, India, 2012

Publications

16. Roy K*, Mitchell S*,Liu Y, Ohta S, Lin YS, Metzig MO, Nutt SL, Hoffmann A. A Regulatory Circuit Controlling the Dynamics of NFκB cRel Transitions B Cells from Proliferation to Plasma Cell Differentiation. Immunity. 2019 Mar 19;50(3):616-628. (*Equal contribution)

15. Mitchell S*, Roy K*, Zangle TA, Hoffmann A. Non-genetic origins of cell-to-cell variability in B-lymphocyte proliferation. Proceedings of the National Academy of Sciences, USA (2018), Mar 7. pii: 201715639. doi: 10.1073/pnas.1715639115. (*Equal contribution)

14. Roy K, Shokhirev M, Mitchell S, Hoffmann A. Deriving quantitative cell biological information from dye-dilution lymphocyte proliferation experiments. Methods in Molecular Biology (2018), 2018;1707:81-94

13. Roy K, Mazumder A, Ghosh P, Naiya G, Ghosh B, Roy S. A Peptide-based Synthetic Transcription Factor Selectively Activates Transcription in a Mammalian Cell. Chemical Communications, 2018, 54(13):1611-1614

12. Roy K*, Mandloi S*, Chakrabarti S, Roy S. Cholesterol Corrects Altered Conformation of MHC-II Protein in Leishmania donovani Infected Macrophages: Implication in Therapy. PLOS Neglected Tropical Diseases, 2016 May 23;10(5):e0004710. (*Equal contribution)

11. Ghosh M*, Roy K*, Das Mukherjee D, Chakrabarti G, Roy Choudhury K, Roy S. Leishmania donovani infection enhances lateral mobility of macrophage membrane protein which is reversed by liposomal cholesterol. PLOS Neglected Tropical Diseases, 2014 Dec4;8(12):e3367. (*Equal contribution)

10. Dhar A, Mallick S, Ghosh P, Maiti A, Ahmed I, Bhattacharya S, Mandal T, Manna A, Roy K, Singh S, Nayak DK, Wilder PT, Markowitz J, Weber D, Ghosh MK, Chattopadhyay S, Guha R, Konar A, Bandyopadhyay S, Roy S. Simultaneous Inhibition of Key Growth Pathways in Melanoma Cells and Tumor Regression by a Designed Bidentate Constrained Helical Peptide. Biopolymers-Peptide Science, 2014 Apr; 101(4):344-58.

9. Roy K, Naskar K, Ghosh M, Roy S. Class II MHC/peptide interaction in Leishmania donovani infection: implications in vaccine design. Journal of Immunology, 2014, 192(12):5873-80.

8. Roy K, Ghosh M, Pal TK, Chakrabarti S, Roy S. Cholesterol lowering drug may influence cellular immune response by altering MHC II function. Journal of Lipid Research, 2013, 54(11):3106-15.
(F1000 Faculty: recommended)

7. Ghosh M, Roy K, Roy S. Immunomodulatory effects of antileishmanial drugs. Journal of Antimicrobial Chemotherapy, 2013, 68(12):2834-8.

6. Ghosh M, Solanki AK, Roy K, Dhoke RR, Ashish, Roy S. Carrier protein influences immunodominance hierarchy: implication in vaccine design. Vaccine, 2013, 31(41):4682-8.

5. Mondal S, Bardhan R, Mondal B, Dey A, Mukhopadhyay SS, Roy S, Guha R, Roy K. Synthesis, characterization and in vitro cytotoxicity assessment of hydroxyapatite from different bioresources for tissue engineering application. Bulletin of Material Science, 2012, 35(4), 683-691.

4. Roy S, Ghosh P, Roy NS, Mazumder A, Roy K, Manna AK, Mallick S, Ahmed I. Peptide based Molecules as Protein-Protein Interaction Inhibitors: Tools for Chemical Genetics and Therapy. Current Chemical Biology, 2012, 6(2):145-163. (Review)

3. Mazumder A, Maiti A, Roy K, Roy S. A synthetic peptide mimic of λ-Cro shows sequence-specific binding in vitro and in vivo. ACS Chemical Biology, 2012, 15;7(6):1084-94.

2. Sen S, Roy K, Mukherjee S, Mukhopadhyay R, Roy S. Restoration of IFNγR subunit assembly, IFNγ signaling and parasite clearance in Leishmania donovani infected macrophages: role of membrane cholesterol. PLoS Pathogen, 2011, September 7(9), e1002229.

1. Raha P, Chattopadhyay S, Mukherjee S, Chattopadhyay R, Roy K, Roy S. Alternative sigma factors in the free state are equilibrium mixtures of open and compact conformations. Biochemistry, 2010, 49, 9809–9819.

TITLE: Graduate Student (PhD)
PROGRAM: Microbiology, Immunology, and Molecular Genetics (MIMG) (2014 – 2019)
EMAIL: kango@nullucla.edu
OFFICE: Boyer Hall 570
PHONE: 310-794-9970
EDUCATION:
M.S. in Chemistry, UC San Diego – 2013
B.S. in Pharmacological Chemistry, UC San Diego – 2008

Biography

My research is focused on understanding the mechanisms underlying the activation of gene transcription by NFκB RelA subunit. This is done by elucidating specific attributes of RelA that contribute to its ability to activate and regulate inflammatory gene expression programs by employing a panel of RelA mutations and examining their impact on transcription. By using biochemical analysis, mathematical model-aided analysis approach, RNA and ChIP-seq analyses, we find specialized, non-overlapping functions in DNA binding and transcriptional activation in RelA. My hobbies include playing beach volleyball, badminton, baking, and volunteering for Buddhist temple events.

Awards and Fellowships

MIMG Warsaw Fellowship, 2017-2018

Sidney Rittenberg Award, 2015

Publications

Fortmann KT, Lewis RD, Ngo KA, Fagerlund R, Hoffmann A. 2015. “A Regulated, Ubiquitin-Independent Degron in IκBα.” Journal of Molecular Biology. PMID: 26191773

Tsui R, Kearns JD, Lynch C, Vu D, Ngo KA, Basak S, Ghosh G, Hoffmann A. 2015. “IκBβ enhances the generation of the low-affinity NFκB/RelA homodimer.” Nature Communications. PMID: 25946967.

Almaden JV, Tsui R, Liu YC, Birnbaum H, Shokhirev MN, Ngo KA, Davis-Turak JC, Otero D, Basak S, Rickert RC, Hoffmann A. 2014. “A pathway switch directs BAFF signaling to distinct NFκB transcription factors in maturing and proliferating B cells”. Cell Reports. PMID: 25497099

Staff Research Associate  & Director of SDCSB Next Gen Gene Expression Analysis Core

birnbaumharry@nullgmail.com
Office: NSB 3209
Phone: 610-764-6695

B.A. Mathematics, University of Pennsylvania – 2012

More about the Next-Gen Gene Expression Core

Publications:

Mukherjee, S.P., Behar, M., Birnbaum, H.A, Hoffmann, A., Wright, P.E., Ghosh, G.
Analysis of the RelA:CBP/p300 interaction reveals its involvement in NFkB-driven transcription
PLOS Biology, 11:e1001647

TITLE: Asst. Project Scientist & Director of Light Microscopy
EMAIL: jadvargas@nullucla.edu
OFFICE: Boyer 570
EDUCATION:
Ph.D. Biology, The Salk Institute | University of California, San Diego – 2012
M.S. Molecular and Cellular Physiology, Southern Illinois University  – 2007
B.S. Biological Sciences, University of California, Irvine – 2003
B.S. Chemistry, University of California, Irvine – 2003

Research Interests

My current research interests are in developing image based assays for mammalian cell signaling systems that utilized live-cell approaches to view signaling events in real time at a single cell level to reveal events and mechanisms obscured by population averaging biochemical approaches. I am interesting in assays with workflows amenable to automation that can be used to generate functional data for the design and validation of mathematical models of gene regulatory networks.

Publications

Hobbies | Activities

In my spare time I try to stay fit doing judo 柔道 or gymnastics or playing volleyball at the beach. I also am a photography enthusiast and spend a lot of time taking random pictures of buildings or landscapes that catch my eye.  I’m a Mac.